The Research Ethics Committee (REC) of the Human Technopole Foundation is responsible for evaluating scientific research projects and activities undertaken at Human Technopole (HT) so that they are ethically reviewed, cleared and monitored in accordance with national and international ethical laws, norms, principles, guidelines, standards and best practices regulating scientific research.In the world of YouTube, where attention is the currency and watch time reigns supreme, content creators are constantly searching for ways to engage their audience and keep them hooked.
HT Research Ethics Committee: open call for external members.Human Technopole’s Centre for Innovation and Technology Transfer (CITT) and Nature Italy are organizing – on 26 and 27 October 2023 – a conference aimed at investigating the new frontiers of research in the life sciences. Future Trends in Translational Medicine.Meet Rodrigo Pracana, Senior Bioinformatician at the Functional Genomics Computation Scientific Service Unit of the Genomics Research Centre – Functional Genomics programme. Exploring HT Research with Rodrigo Pracana from Genomics.The Pigino Group presents a CRISPR/Cas-based protocol for the targeted insertion of exogenous genes in the unicellular green alga Chlamydomonas that is amenable to rapid high-throughput screening of mutant cells. Knocking-in Chlamydomonas reinhardtii genes with CRISPR/Cas.Following 25 years in Dresden where he served as Director of the Max Planck Institute of Molecular Cell Biology and Genetics, Marino was appointed as Human Technopole Director in February 2023 by the On 1 September 2023 the Human Technopole community warmly welcomed Marino Zerial as he officially took office as the institute’s new Director. Welcome to our new Director Marino Zerial.(2022) In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains. The Pigino Group has already used this protocol to study the assembly of intraflagellar transport trains (IFT) at the Chlamydomonas ciliary base 1, thus showing that this new procedure will be instrumental for researchers to perform super-resolution analysis of target proteins in a native 3D environment without the need for expensive super-resolution microscopes and specific training.ġ van den Hoek et al.
Linkedin dark mode how to#
In this protocol, the researchers compare different fixation and staining procedures and provide instructions on how to embed the sample in a hydrogel and acquire images with a confocal microscope as well as how to analyse them. The protocol is now published in the open-access journal Bio-protocol and made available to the research community.Ĭhlamydomonas is a single-cell model organism widely used to investigate the molecular mechanisms of cilia and flagella motility.
Gaia Pigino, Associate Head of the Structural Biology Research Centre at Human Technopole, Nikolai Klena and Giovanni Maltinti, members of the Pigino Group, in collaboration with Paul Guichard and Virginie Hamel at the University of Geneve, developed an ExM-based protocol to visualise the three-dimensional (3D) ultrastructural organisation of Chlamydomonas reinhardtii (Ultrastructure Expansion Microscopy, U-ExM). Expansion Microscopy (ExM) has recently been developed to overcome these limitations and uses swellable hydrogels to obtain up to fourfold isotropic expansion of the sample before imaging with a confocal microscope.
However, super-resolution microscopes are usually expensive instruments with complicated setups and require experienced personnel for sample preparation and image acquisition. The advent of super-resolution imaging techniques coupled with fluorescence microscopy allowed the visualisation of a vast array of biological samples with a resolution higher than that imposed by the light diffraction limit. The Pigino Group developed a detailed protocol to obtain super-resolution images of the green alga Chlamydomonas reinhardtii by using conventional optical light microscopes.
0 kommentar(er)
